中新网广州9月5日电(杨薇 朱丹萍)笔者5日从中科院广州生物医药与健康研究院获悉，该院研发出一种可区别单核苷酸多态性(SNP)的核酸检测生物传感器。

Beijing on September 5, guangzhou(YangWei ZhuDanPing)The author 5, the Chinese academy of sciences from guangzhou biological medicine and health research institute learn,The hospital developed a can be the difference between single nucleotide polymorphisms(SNP)The nucleic acid detection biosensor.

　　该研究是由中科院广州生物医药与健康研究院曾令文研究组完成，他们研发出一种基于核酸等温链置换反应技术、T4连接酶反应与胶体金技术的可区别单核苷酸多态性的核酸检测生物传感器。

The study is from the Chinese academy of sciences guangzhou biological medicine and health research institute CengLingWen the team completed,They developed a based on nucleic acid isothermal chain substitution reaction technology/T4 connection enzyme reaction and colloidal gold technology can be the difference between single nucleotide polymorphism of nucleic acid detection biosensor.

　　据该课题组研发人员介绍，这一过程简单说就是取两滴血，经过核酸提取后，与检测试剂室温反应半小时后滴加入试纸条上5分钟后即可观察到结果，如果有单核苷酸多态性引起的遗传病、肿瘤疾病的易感基因存在或病原微生物，检测线就会显现出来，没有则不显示。

According to the research and development personnel introduces research,This process is simple said take two blood,After the nucleic acid after extraction,And detection reagent room temperature reaction after half an hour on drop join dipsticks 5 minutes and then observed results,If there is a single nucleotide polymorphism cause genetic disease/Tumor disease susceptibility genes exist or pathogenic microorganism,Detection line will come out,No, don't show.

　　基于该检测方法，他们已开发了的应用包括有：输血四项病原体的检测，地中海贫血基因突变的检测。

Based on the test method,They have developed applications include have:Blood transfusion four pathogen detection,Mediterranean anemia gene mutation detection.

　　曾令文强调，单核苷酸多态性的检测非常重要，很多遗传疾病或肿瘤疾病均由单核苷多态性引起的基因，当检测到阳性结果时，表明被检者携带潜在的致病基因。虽然目前还不一定发病，但医生可以提醒被检者，注意生活方式或采取一些预防方法。

CengLingWen emphasize,Single nucleotide polymorphisms of the detection is very important,A lot of genetic diseases or tumor diseases are caused by listeria glycosides polymorphism gene,When detected positive results,That was examiner carry potential pathogenic gene.Although there is not necessarily disease,But doctors can remind be examiner,Caution that lifestyle or take some preventive methods.

　　单核苷酸多态性(SNP)主要是指在基因组水平上由单个核苷酸的变异所引起的DNA序列多态性。它是人类可遗传的变异中最常见的一种。占所有已知多态性的90%以上，是DNA变异中最常见的一种。

Single nucleotide polymorphism(SNP)Mainly refers to the genome level by single nucleotide mutation caused by the DNA sequence polymorphism.It is a human can genetic variation is the most common one.Accounted for more than 90% of all known polymorphism,DNA variation is the most common one.

　　“以往的单核苷酸多态性检测的方法，需要的仪器比较复杂昂贵，一台仪器在几万到几十万元，检测时间也较长，需要一两个小时，甚至更长。”曾令文介绍，他们研发出的这种新型的生物传感器，不需要复杂的检测仪器，仅需室温反应，其灵敏度高，单次反应可检测约6个核酸分子，此外它的特异性也很高，可区分单碱基突变。他还称，这种检测方法在“半小时就能显现结果”，比以往的检测方式便宜了很多，不但医院能使用，将来家庭也可能使用。

"Previous single nucleotide polymorphism testing method,Need more complex expensive instruments,One instrument to hundreds of thousands of dollars in tens,Testing time is long,Need a two hours,Even more."CengLingWen introduced,They developed this kind of new type of biosensor,Don't need complex testing instrument,Only room temperature response,Its high sensitivity,Single reaction can test about six nucleic acid molecule,In addition, it is also high specificity,Can distinguish single base mutation.He also says,This kind of detection method in"Half an hour can appear the results",Detecting way than in the past a lot cheaper,Not only the hospital can use,The future family may also use.

　　曾令文表示，希望该检测方法今后家庭也可以方便的使用，他认为，只要解决了核酸的提取的问题，该检测技术应用在家庭中完全有可能。这样有一天，市民能在家里使用这种试纸条，方便的检测自己是否有遗传病、肿瘤基因或传染性疾病，从而做出相应的健康防范与治疗。(完)

CengLingWen said,Hope this detection method in the future family can also be convenient to use,He thinks,As long as solved the problem of DNA extraction,This testing technique used in the family, it is entirely possible.So one day,People can use in the home the dipsticks,Convenient testing whether oneself have genetic disease/Tumor gene or infectious diseases,Thus makes the corresponding health prevention and treatment.(finish)